肿瘤抑制因子NPRL2在肝细胞癌的进展中起重要作用，可作为独立的预后因素。

The tumor suppressor NPRL2 in hepatocellular carcinoma plays an important role in progression and can be served as an independent prognostic factor.

作者信息

Otani Satoshi, Takeda Shin, Yamada Suguru, Sakakima Yoshikazu, Sugimoto Hiroyuki, Nomoto Shuji, Kasuya Hideki, Kanazumi Naohito, Nagasaka Tetsurou, Nakao Akimasa

机构信息

Department of Surgery II, Graduate School of Medicine, Nagoya University, Nagoya, Japan.

出版信息

J Surg Oncol. 2009 Oct 1;100(5):358-63. doi: 10.1002/jso.21241.

DOI:10.1002/jso.21241

PMID:19274676

Abstract

BACKGROUND/AIMS: Hepatocarcinogenesis is a multifactorial, multistep process that involves the activation of oncogenes or the inactivation of tumor suppressor genes throughout the different stages of hepatocellular carcinoma (HCC) progression. NPRL2 is one of the candidate tumor suppressor genes identified on chromosome 3p21.3, a region which frequently contains genetic abnormalities found in the early stages of the development of various human cancers. In the current study, we aimed to evaluate NPRL2 expression in HCC and to explore the prognostic significance of NPRL2.

METHOD

We investigated NPRL2 mRNA expression in 70 HCC specimens, using quantitative real-time reverse transcription polymerase chain reaction analysis, and the correlation between NPRL2 expression and clinicopathologic parameters.

RESULTS

NPRL2 mRNA was found to be expressed equally in both HCC tissues and corresponding non-cancerous liver tissues. However, higher NPRL2 expression correlated significantly with tumor size (P = 0.0062) and serum PIVKA-II levels (P = 0.0002). Univariate and multivariate analyses revealed that higher NPRL2 mRNA expression was an independent prognostic factor for overall survival (risk ratio 0.39; P < 0.0001).

CONCLUSION

Our results suggest that NPRL2 mRNA expression has prognostic significance for the survival of patients with HCC.

摘要

背景/目的：肝癌发生是一个多因素、多步骤的过程，在肝细胞癌（HCC）进展的不同阶段涉及癌基因的激活或肿瘤抑制基因的失活。NPRL2是在3p21.3染色体上鉴定出的候选肿瘤抑制基因之一，该区域在各种人类癌症发展的早期阶段经常存在基因异常。在本研究中，我们旨在评估NPRL2在肝癌中的表达，并探讨NPRL2的预后意义。

方法

我们使用定量实时逆转录聚合酶链反应分析研究了70例肝癌标本中NPRL2 mRNA的表达，以及NPRL2表达与临床病理参数之间的相关性。

结果

发现NPRL2 mRNA在肝癌组织和相应的非癌肝组织中表达相当。然而，较高的NPRL2表达与肿瘤大小（P = 0.0062）和血清异常凝血酶原水平（P = 0.0002）显著相关。单因素和多因素分析显示，较高的NPRL2 mRNA表达是总生存期的独立预后因素（风险比0.39；P < 0.0001）。