Nicolau José, De Souza Douglas Nesadal, Simões Alyne
Oral Biology Research Center, University of São Paulo, Brazil.
Cell Biochem Funct. 2009 Apr;27(3):128-34. doi: 10.1002/cbf.1544.
Diabetes has been implicated in the dryness of the mouth, loss of taste sensation, sialosis, and other disorders of the oral cavity, by impairment of the salivary glands. The aim of the present study was to examine the plasma membrane, microsomal, and homogenate Ca(2+)-ATPase activity in the rat submandibular and parotid salivary glands of streptozotocin-induced diabetes. We have also examined the influence of the acidosis state on this parameter. Diabetes was induced by an intraperitoneal injection of streptozotocin and acidosis was induced by daily injection of NH(4)Cl. At 15 and 30 days after diabetes induction, the animals were euthanized and the submandibular and parotid salivary glands were removed and analyzed. Ca(2+)-ATPase (total, independent, and dependent) was determined in the homogenate, microsomal, and plasma membranes of the salivary glands of diabetic and control rats. Calcium concentration was also determined in the glands and showed to be higher in the diabetic animals. Ca(2+)-ATPase activity was found to be reduced in all cell fractions studied in the diabetic animals compared with control. Similar results were obtained for the submandibular salivary glands of acidotic animals; however in the parotid salivary glands it was found an increase in the enzyme activity.
糖尿病与口干、味觉丧失、涎腺肿大及其他口腔疾病有关，原因是唾液腺功能受损。本研究的目的是检测链脲佐菌素诱导的糖尿病大鼠下颌下腺和腮腺的质膜、微粒体及匀浆中Ca(2+)-ATP酶活性。我们还研究了酸中毒状态对该参数的影响。通过腹腔注射链脲佐菌素诱导糖尿病，通过每日注射NH(4)Cl诱导酸中毒。在诱导糖尿病后15天和30天，处死动物，取出下颌下腺和腮腺并进行分析。测定糖尿病大鼠和对照大鼠唾液腺匀浆、微粒体及质膜中Ca(2+)-ATP酶（总量、独立型和依赖型）。还测定了腺体中的钙浓度，结果显示糖尿病动物的钙浓度更高。与对照相比，糖尿病动物所有研究的细胞组分中Ca(2+)-ATP酶活性均降低。酸中毒动物的下颌下腺也得到了类似结果；然而，腮腺中该酶活性增加。
