酸性高锰酸钾化学发光流动注射法测定药物中的视黄醇和生育酚

Flow-injection determination of retinol and tocopherol in pharmaceuticals with acidic potassium permanganate chemiluminescence.

作者信息

Waseem Amir, Rishi Lubna, Yaqoob Mohammad, Nabi Abdul

机构信息

Department of Chemistry, University of Balochistan, Quetta, Pakistan.

出版信息

Anal Sci. 2009 Mar;25(3):407-12. doi: 10.2116/analsci.25.407.

DOI:10.2116/analsci.25.407

PMID:19276599

Abstract

A simple and rapid flow-injection method is reported for the determination of retinol, its derivatives and alpha-tocopherol by its enhancement effect on a potassium permanganate-formaldehyde chemiluminescence system in an acidic medium. Linear calibration graphs were obtained between 5.0 x 10(-8) to 5.0 x 10(-6) mol L(-1) for all-trans-retinol, 5.0 x 10.8 - 2.5 x 10(-6) mol L(-1) for all-trans-retinal, 5.0 x 10(-7) - 1.0 x 10.5 mol L(-1) for retinoic acid, 1.0 x 10(-7) - 5.0 x 10(-6) mol L(-1) for retinyl acetate and retinyl palmitate and 1.0 x 10(-8) - 5.0 x 10(-6) for alpha-tocopherol, with relative standard deviations (RSD; n = 4) in the range of 0.8 - 2.8%. The limits of detection (S/N = 3) were in the range of 5.0 x 10(-9) - 1.0 x 10(-7) mol L(-1) with a sample throughput of 100 h(-1). The influence of common ions, excipients in pharmaceutical preparations and related organic compounds were studied on the determination of retinol and alpha-tocopherol individually. The method was applied to determine retinol and alpha-tocopherol in pharmaceutical preparations, and the results obtained were in reasonable agreement with the amount labelled.

摘要

报道了一种简单快速的流动注射方法，用于在酸性介质中通过其对高锰酸钾 - 甲醛化学发光体系的增强作用来测定视黄醇、其衍生物和α-生育酚。全反式视黄醇在5.0×10⁻⁸至5.0×10⁻⁶ mol L⁻¹之间、全反式视黄醛在5.0×10⁻⁸至2.5×10⁻⁶ mol L⁻¹之间、视黄酸在5.0×10⁻⁷至1.0×10⁻⁵ mol L⁻¹之间、醋酸视黄酯和棕榈酸视黄酯在1.0×10⁻⁷至5.0×10⁻⁶ mol L⁻¹之间以及α-生育酚在1.0×10⁻⁸至5.0×10⁻⁶ mol L⁻¹之间获得了线性校准曲线，相对标准偏差（RSD；n = 4）在0.8 - 2.8%范围内。检测限（S/N = 3）在5.0×10⁻⁹至1.0×10⁻⁷ mol L⁻¹范围内，进样量为100 h⁻¹。分别研究了常见离子、药物制剂中的辅料和相关有机化合物对视黄醇和α-生育酚测定的影响。该方法用于测定药物制剂中的视黄醇和α-生育酚，所得结果与标示量合理一致。