用于聚糖结构解析的碰撞诱导解离串联质谱法。

Collision-induced dissociation tandem mass spectrometry for structural elucidation of glycans.

作者信息

Li Bensheng, An Hyun Joo, Hedrick Jerry L, Lebrilla Carlito B

机构信息

Department of Chemistry, University of California, Davis, CA, USA.

出版信息

Methods Mol Biol. 2009;534:133-45. doi: 10.1007/978-1-59745-022-5_10.

DOI:10.1007/978-1-59745-022-5_10

PMID:19277555

Abstract

The complexity of glycans poses a major challenge for structure elucidation. Tandem mass spectrometry is currently an efficient and powerful technique for the structural characterization of glycans. Collision-induced dissociation (CID) is most commonly used, and involves first isolating the glycan ions of interest, translationally exciting them, and then striking them with inert target gas to fragment the precursor ions. The structural information of the glycan can be obtained from the fragment ions of the tandem MS spectra. In this chapter, sustained off-resonance irradiation-collision-induced dissociation (SORI-CID) implemented with matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI FT ICR MS) is demonstrated to be a useful analysis tool for structural elucidation of mucin-type O-glycans released from mucin glycoproteins. The mechanisms by which the glycans undergo fragmentations in the tandem mass analysis are also discussed.

摘要

聚糖的复杂性给结构解析带来了重大挑战。串联质谱法是目前用于聚糖结构表征的一种高效且强大的技术。碰撞诱导解离（CID）是最常用的方法，它首先分离出感兴趣的聚糖离子，使其产生平动激发，然后用惰性目标气体撞击它们以使前体离子碎片化。聚糖的结构信息可从串联质谱图的碎片离子中获得。在本章中，采用基质辅助激光解吸/电离傅里叶变换离子回旋共振质谱（MALDI FT ICR MS）实现的持续非共振照射碰撞诱导解离（SORI-CID）被证明是用于解析从粘蛋白糖蛋白释放的粘蛋白型O-聚糖结构的一种有用分析工具。同时还讨论了聚糖在串联质谱分析中发生碎片化的机制。