Harvey David J
Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford, OX1 3QU, UK.
Mass Spectrom Rev. 2015 May-Jun;34(3):268-422. doi: 10.1002/mas.21411. Epub 2014 May 26.
This review is the sixth update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2010. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, arrays and fragmentation are covered in the first part of the review and applications to various structural typed constitutes the remainder. The main groups of compound that are discussed in this section are oligo and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals. Many of these applications are presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions and applications to chemical synthesis.
本综述是1999年发表的关于基质辅助激光解吸电离质谱（MALDI）在碳水化合物和糖缀合物分析中的应用的原始文章的第六次更新，涵盖了截至2010年底的文献。综述的第一部分涵盖了MALDI过程的理论、基质、衍生化、MALDI成像、阵列和碎片化等一般方面，其余部分则是各种结构类型的应用。本节讨论的主要化合物类别包括寡糖和多糖、糖蛋白、糖脂、糖苷和生物制药。其中许多应用以表格形式呈现。还讨论了该技术的医学和工业应用、酶反应研究以及在化学合成中的应用。
