桥粒芯糖蛋白酶联免疫吸附测定在天疱疮诊断中的应用及其与寻常型天疱疮严重程度的相关性
Desmoglein ELISA in the diagnosis of pemphigus and its correlation with the severity of pemphigus vulgaris.
作者信息
Mortazavi Hossein, Shahdi Majid, Amirzargar Ali Akbar, Naraghi Zahra S, Valikhani Mahin, Daneshpazhooh Maryam, Vasheghani-Farahani Amir, Sedaghat Mojtaba, Chams-Davatchi Cheyda
机构信息
Department of Dermatology, Autoimmune Bullous Diseases Research Center, School of Medicine, Tehran University of Medical Sciences, Tehran Iran.
出版信息
Iran J Allergy Asthma Immunol. 2009 Mar;8(1):53-6.
Anti-desmoglein 3 and 1 autoantibodies are involved in the pathogenesis of pemphigus diseases. Our objective was to assess the value of ELISA in the diagnosis of pemphigus and its correlation with the severity of pemphigus vulgaris. Based on clinical presentation and histopathologic confirmation for the diagnosis of the pemphigus, 38 patients took part in the study. Sera of the patients were tested by desmoglein 1 and desmoglein 3 ELISA. Also, direct immunofluorescence was performed for all patients which revealed positive results in 36 patients (94.7%). ELISA was positive in 37 of 38 pemphigus patients (Sensitivity: 97.3%). The relationship between desmoglein 1 index values and skin severity was statistically significant (p<0.05). Desmoglein 3 index values increased with oral severity although this was not statistically significant. Iranian patients similar to Indian patients had higher positive anti-desmoglein 1 autoantibodies. Desmoglein-ELISA test is appropriate in the diagnosis of pemphigus. Desmoglein 1 index value is statistically correlated with the severity of pemphigus vulgaris.
抗桥粒芯糖蛋白3和1自身抗体参与天疱疮疾病的发病机制。我们的目的是评估酶联免疫吸附测定（ELISA）在天疱疮诊断中的价值及其与寻常型天疱疮严重程度的相关性。基于临床表现和组织病理学确诊天疱疮，38例患者参与了该研究。采用桥粒芯糖蛋白1和桥粒芯糖蛋白3 ELISA检测患者血清。此外，对所有患者进行直接免疫荧光检查，结果显示36例患者（94.7%）呈阳性。38例天疱疮患者中有37例ELISA呈阳性（敏感性：97.3%）。桥粒芯糖蛋白1指数值与皮肤严重程度之间的关系具有统计学意义（p<0.05）。桥粒芯糖蛋白3指数值随口腔严重程度增加，尽管这无统计学意义。与印度患者相似，伊朗患者抗桥粒芯糖蛋白1自身抗体阳性率更高。桥粒芯糖蛋白ELISA检测适用于天疱疮的诊断。桥粒芯糖蛋白1指数值与寻常型天疱疮的严重程度具有统计学相关性。
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