Iino Satoshi, Horiguchi Kazuhide, Horiguchi Satomi, Nojyo Yoshiaki
Department of Morphological and Physiological Sciences, University of Fukui Faculty of Medical Sciences, Eiheiji, Fukui 910-1193, Japan.
Histochem Cell Biol. 2009 Jun;131(6):691-702. doi: 10.1007/s00418-009-0580-6. Epub 2009 Mar 11.
Platelet-derived growth factor receptors (PDGFRs) belong to the same kinase group as c-Kit receptor tyrosine kinase that is specifically expressed in the interstitial cells of Cajal (ICC) in the gastrointestinal tract. In this study, we examined PDGFRalpha immunoreactivity in the murine gastrointestinal tract. PDGFRalpha-immunopositive (PDGFRalpha-ip) cells were observed in the musculature in all parts of the gastrointestinal tract. Although PDGFRalpha-ip cells were distinct from ICC and neurons, these cells were closely associated with intramuscular ICC and enteric nerve fibers. In the myenteric layer, PDGFRalpha-ip cells formed a cellular network with their ramified processes and encompassed myenteric ganglia. Numerous PDGFRalpha-ip cells were observed in the subserosal plane and showed a multipolar shape. The distribution pattern of the PDGFRalpha-ip cells in the ICC-deficient W(v)/W(v) mutant mice was the same as that in normal mice. PDGFRalpha-ip cells that showed intense immunoreactivity of SK3 potassium channel were considered to correspond to fibroblast-like cells or non-Cajal interstitial cells. Our observations suggest that PDGFRalpha-ip cells are basic cellular elements throughout the gastrointestinal musculature and are involved in the gastrointestinal functions.
血小板衍生生长因子受体（PDGFRs）与c-Kit受体酪氨酸激酶属于同一激酶家族，c-Kit受体酪氨酸激酶在胃肠道的 Cajal 间质细胞（ICC）中特异性表达。在本研究中，我们检测了小鼠胃肠道中PDGFRα的免疫反应性。在胃肠道各部位的肌肉组织中均观察到PDGFRα免疫阳性（PDGFRα-ip）细胞。尽管PDGFRα-ip细胞与ICC和神经元不同，但这些细胞与肌内ICC和肠神经纤维密切相关。在肌间层，PDGFRα-ip细胞通过其分支状突起形成细胞网络，并包围肌间神经节。在浆膜下层观察到大量PDGFRα-ip细胞，呈多极形状。ICC缺陷的W(v)/W(v)突变小鼠中PDGFRα-ip细胞的分布模式与正常小鼠相同。显示SK3钾通道强烈免疫反应性的PDGFRα-ip细胞被认为对应于成纤维细胞样细胞或非Cajal间质细胞。我们的观察结果表明，PDGFRα-ip细胞是整个胃肠道肌肉组织中的基本细胞成分，并参与胃肠道功能。
