Department of Semen Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747, Olsztyn, Poland.
Department of Ichthyology, Warmia and Mazury University, 10-718, Olsztyn-Kortowo, Poland.
Fish Physiol Biochem. 2010 Sep;36(3):419-425. doi: 10.1007/s10695-009-9311-0. Epub 2009 Mar 12.
The objective of this study was to describe the morphometry and motility parameters of vendace (Coregonus albula) spermatozoa. Morphometric parameters of vendace sperm head and tail were of values similar to rainbow trout. The effects of pH, sodium, potassium and calcium ion concentrations on computer-assisted sperm analysis (CASA) sperm motility characteristics were tested. Vendace sperm was motile in a wide pH range of 6.0-10.5 with the optimum pH established at 9.0. Increases in potassium and calcium ions caused decreases in the percentage of motile sperm. The CASA parameters and erratic sperm movement pattern of vendace spermatozoa were similar to whitefish (C. lavaretus) sperm motility, suggesting that there is a coregonid-specific sperm motility pattern.
本研究的目的是描述白鲑(Coregonus albula)精子的形态和运动参数。白鲑精子头和尾的形态参数与虹鳟相似。测试了 pH 值、钠离子、钾离子和钙离子浓度对计算机辅助精子分析(CASA)精子运动特性的影响。白鲑精子在 pH 值为 6.0-10.5 的较宽范围内具有运动性,最佳 pH 值为 9.0。钾离子和钙离子的增加导致运动精子的百分比下降。CASA 参数和白鲑精子的不稳定运动模式与白鱼(C. lavaretus)精子运动相似,表明存在一种特定于白鲑科的精子运动模式。