Swinburne T R
Department of Agriculture for Northern Ireland, Queen's University, Belfast, UK.
Ann Appl Biol. 1974 Sep;78(1):39-48. doi: 10.1111/j.1744-7348.1974.tb01483.x.
The log of the time interval between inoculation with Nectria galligena in October and the onset of rotting in apples held in air was proportional to the deficit between the temperature of incubation and 25 degrees C, but temperature did not affect the rate of subsequent rot expansion. Rots expanded equally fast whether apples were held in dry or moist air. The quantity of rotted tissue obtained after incubating inoculated apples in atmospheres containing up to 12.5% CO2 increased with increasing concentrations of CO2 greater than 2.5%. The quantity of rotted tissue obtained in apples incubated in 10% CO2 was three times as great as that obtained after incubation in air. The incidence of natural rots was lower in apples stored at 4% CO2 than in those stored in air and rotting increased with increasing concentrations of CO2 higher than 4%. Colonies of N. galligena grew faster on malt agar plates incubated in 5% CO2 than in air, but growth was slower in 10% CO2 than in air. The quantity of benzoic acid per mg hyphae accumulated in developing lesions was similarly related to the CO2 concentrations up to 2.5% but decreased at higher concentrations, and the quantities found in apples stored in CO2 concentrations > 5.0% CO2 were less than in those stored in air.
10月接种苹果黑腐病菌（Nectria galligena）至苹果在空气中开始腐烂的时间间隔的对数，与培养温度和25摄氏度之间的差值成正比，但温度并不影响随后腐烂扩展的速率。无论苹果保存在干燥还是潮湿的空气中，腐烂扩展速度相同。在含有高达12.5%二氧化碳的气氛中培养接种后的苹果，腐烂组织的量随二氧化碳浓度高于2.5%而增加。在10%二氧化碳中培养的苹果所获得的腐烂组织量是在空气中培养后获得量的三倍。保存在4%二氧化碳中的苹果自然腐烂发生率低于保存在空气中的苹果，且腐烂率随二氧化碳浓度高于4%而增加。苹果黑腐病菌在5%二氧化碳中培养的麦芽琼脂平板上生长速度比在空气中快，但在10%二氧化碳中比在空气中慢。在发育中的病斑中每毫克菌丝积累的苯甲酸量与二氧化碳浓度在2.5%以下时同样相关，但在较高浓度时减少，且保存在二氧化碳浓度>5.0%的苹果中的苯甲酸量低于保存在空气中的苹果。
