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胞质60 kDa孕酮受体亚型在足月时的人羊膜绒毛膜和胎盘中占主导地位。

The cytoplasmic 60 kDa progesterone receptor isoform predominates in the human amniochorion and placenta at term.

作者信息

Taylor Anthony H, McParland Penny C, Taylor David J, Bell Stephen C

机构信息

Preterm Birth Research Group, Reproductive Sciences, Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, Leicestershire, LE2 7LX, UK.

出版信息

Reprod Biol Endocrinol. 2009 Mar 13;7:22. doi: 10.1186/1477-7827-7-22.

DOI:10.1186/1477-7827-7-22
PMID:19284643
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2669089/
Abstract

BACKGROUND

The mechanism that initiates human parturition has been proposed to be 'functional progesterone withdrawal' whereby the 116 kDa B-isoform of the progesterone receptor (PR-B) switches in favour of the 94 kDa A-isoform (PR-A) in reproductive tissues. Recently, other PR isoforms, PR-S, PR-C and PR-M generated from the same gene have been identified and partially characterised.

METHODS AND RESULTS

Using immunohistochemical, western blotting and RT-PCR techniques, evidence is provided that indicates the major PR isoform present in human term fetal membranes (amnion and chorion) and syncytiotrophoblast of the placenta is neither of the classical nuclear PR-B or PR-A isoforms but is the N-terminally truncated 60 kDa PR-C isoform. Evidence is also provided that this 60 kDa isoform resides in the cytoplasm of the expressing cell types. Data are also presented to show that PR-B, PR-A and PR-S isoforms are essentially absent from the amnion and chorion, whereas PR isoforms A, B, C and S are all present in the decidua, with PR-A being the major isoform. The syncytiotrophoblast of the placenta contains the cytoplasmic 60 kDa isoform, but not isoforms PR-A, PR-B or PR-S.

CONCLUSION

The major PR isoform in the amnion, chorion and placenta is a 60 kDa protein that could be PR-C, suggesting that the cytoplasmic isoform has a specific role in extra-embryonic tissues and may be involved in the regulation of human parturition.

摘要

背景

引发人类分娩的机制被认为是“功能性孕酮撤退”，即生殖组织中孕酮受体（PR）的116 kDa B亚型转变为94 kDa A亚型（PR-A）。最近，已鉴定并部分表征了由同一基因产生的其他PR亚型，PR-S、PR-C和PR-M。

方法与结果

使用免疫组织化学、蛋白质印迹和逆转录聚合酶链反应技术，提供的证据表明，足月人胎膜（羊膜和绒毛膜）和胎盘合体滋养层中存在的主要PR亚型既不是经典的核PR-B或PR-A亚型，而是N端截短的60 kDa PR-C亚型。还提供了证据表明这种60 kDa亚型存在于表达细胞类型的细胞质中。数据还表明，羊膜和绒毛膜中基本不存在PR-B、PR-A和PR-S亚型，而PR-A、B、C和S亚型均存在于蜕膜中，其中PR-A是主要亚型。胎盘合体滋养层含有细胞质60 kDa亚型，但不含有PR-A、PR-B或PR-S亚型。

结论

羊膜、绒毛膜和胎盘中的主要PR亚型是一种60 kDa的蛋白质，可能是PR-C，这表明细胞质亚型在胚外组织中具有特定作用，可能参与人类分娩的调节。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38c/2669089/1dd907a6e3da/1477-7827-7-22-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38c/2669089/87287d3ecd3e/1477-7827-7-22-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38c/2669089/3ca83b583f4f/1477-7827-7-22-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38c/2669089/85f582205a57/1477-7827-7-22-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38c/2669089/8b1229fd5f9a/1477-7827-7-22-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38c/2669089/1dd907a6e3da/1477-7827-7-22-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38c/2669089/87287d3ecd3e/1477-7827-7-22-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38c/2669089/3ca83b583f4f/1477-7827-7-22-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38c/2669089/85f582205a57/1477-7827-7-22-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38c/2669089/8b1229fd5f9a/1477-7827-7-22-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38c/2669089/1dd907a6e3da/1477-7827-7-22-5.jpg

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