Institute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, China.
Comp Biochem Physiol B Biochem Mol Biol. 2009 May;153(1):43-53. doi: 10.1016/j.cbpb.2009.01.008. Epub 2009 Jan 22.
Calcineurin (CN), consisting of catalytic subunit (CN A) and regulatory subunit (CN B), is a multifunctional protein involved in many important physiological processes. Here, we cloned two subunits of CN (Pf-CN A and Pf-CN B) from pearl oyster Pinctada fucata and reported, for the first time, its expression patterns in the developmental stages, its enzymatic activity and immunolocalization in various tissues of adult pearl oyster. The Pf-CN A was extensively localized in all the tested tissues including mantle, gonad, digestive gland, gills, adductor muscle, and foot with strong signals detected in gonad, gills, foot, and mantle. Importantly, Pf-CN A was mainly found in the inner epithelial cells of the basal periostracal groove and lateral surface of the inner mantle fold, in which organic macromolecules used for periostracum formation and shell construction are secreted, respectively. In gill, the strong signals were distributed in the epithelial cells of the branchial filaments and the base of gill filaments. All the results suggested that Pf-CN may participate in the development of the pearl oyster and function in many ways in various physiological activities, especially in the shell formation. Our observations could provide some important clues to further understanding of the functions of CN in the oyster.
钙调神经磷酸酶（CN）由催化亚基（CN A）和调节亚基（CN B）组成，是一种参与许多重要生理过程的多功能蛋白。本研究从珍珠贝（Pinctada fucata）克隆了 CN 的两个亚基（Pf-CN A 和 Pf-CN B），并首次报道了其在发育阶段的表达模式、在成年珍珠贝各组织中的酶活性和免疫定位。Pf-CN A 广泛存在于所有检测组织中，包括套膜、性腺、消化腺、鳃、闭壳肌和足部，在性腺、鳃、足部和套膜中检测到强烈的信号。重要的是，Pf-CN A 主要存在于基围角质层凹槽的内上皮细胞和内套膜褶皱的外侧表面，分别用于分泌形成角质层和壳结构的有机大分子。在鳃中，强烈的信号分布在鳃丝的上皮细胞和鳃丝基部。所有结果表明，Pf-CN 可能参与珍珠贝的发育，并在多种生理活动中发挥多种功能，特别是在壳形成过程中。我们的观察结果可为进一步了解 CN 在牡蛎中的功能提供一些重要线索。
