Hino Shingo, Matsubara Takeshi, Urisu Atsuo, Aoki Naohito, Sato Chihiro, Okajima Tetsuya, Nadano Daita, Matsuda Tsukasa
Department of Applied Molecular Biosciences, Graduate School of Bio-agricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.
Biochem Biophys Res Commun. 2009 Mar 13;380(3):632-7. doi: 10.1016/j.bbrc.2009.01.138. Epub 2009 Jan 27.
The beta(1,2)-xylose- and/or alpha(1,3)-fucose-containing cross-reactive carbohydrate determinants (CCDs) are present in various plant and insect N-glycans, and have been attracted as potential antigens in IgE-mediated allergies and immunologically undesired post-translational products on some recombinant therapeutic proteins. By using ELISA and immunoblotting, CCDs-specific IgG and IgE antibodies from some, but not all, of mice and humans were found to fully retain their binding activity after a typical periodate-treatment to CCDs, which did cause the CCDs' antigenic activity to those from the other mice and rabbits to disappear almost completely. Furthermore, the mouse IgE recognizing the periodate-resistant CCDs induced the CCDs/IgE-dependent degranulation of rat basophilic RBL-2H3 cells. These findings indicate that in some cases CCDs include those dependent of the core trisaccharide more strongly than the terminal xylose and fucose, which might have been screened out in the CCDs analyses based on the loss of antibody-binding by the periodate-treatment.
含有β(1,2)-木糖和/或α(1,3)-岩藻糖的交叉反应性碳水化合物决定簇（CCDs）存在于各种植物和昆虫的N-聚糖中，并且在IgE介导的过敏反应中作为潜在抗原以及在一些重组治疗性蛋白质上作为免疫上不需要的翻译后产物受到关注。通过使用酶联免疫吸附测定（ELISA）和免疫印迹法，发现部分（而非全部）小鼠和人类的CCDs特异性IgG和IgE抗体在经过典型的高碘酸盐处理后，对CCDs仍能完全保留其结合活性，而这种处理确实使CCDs对其他小鼠和兔子的抗原活性几乎完全消失。此外，识别耐高碘酸盐CCDs的小鼠IgE诱导了大鼠嗜碱性RBL-2H3细胞的CCDs/IgE依赖性脱粒。这些发现表明，在某些情况下，CCDs中包含那些比末端木糖和岩藻糖更强烈依赖核心三糖的成分，而这些成分可能在基于高碘酸盐处理导致抗体结合丧失的CCDs分析中被筛选掉了。
