Sun Fenyong, Wang Jiayi, Pan Qiuhui, Yu Yongchun, Zhang Yue, Wan Yang, Wang Ju, Li Xiaoyan, Hong An
Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou City, 510632 Guangdong Province, PR China.
Biochem Biophys Res Commun. 2009 Mar 13;380(3):660-5. doi: 10.1016/j.bbrc.2009.01.161. Epub 2009 Jan 31.
To date, numerous microRNAs (miRNAs) have been discovered. However, the function of these miRNAs is largely unknown. While our knowledge of miRNA post-transcriptional processing has greatly expanded in recent years, we have a limited understanding of the regulation and transcription of miRNA genes. In this study, we characterized two BMP-2 upregulated miRNAs, miR-24-1 and miR-31, in mesenchymal stem cells and showed their opposing function in controlling cellular proliferation, and adipogenesis. Furthermore, we are the first to identify and characterize mouse intronic miR-23b27b24-1 and intergenic miR-31 genes. Moreover, we found that pri-miR-23b, pri-miR-27b, and pri-miR-24-1 are transcribed independently and their expression profiles are unique when cells are treated with BMP-2, even though they are located closely together.
迄今为止，已发现了众多微小RNA（miRNA）。然而，这些miRNA的功能在很大程度上尚不清楚。尽管近年来我们对miRNA转录后加工的了解有了极大扩展，但对于miRNA基因的调控和转录，我们的认识仍有限。在本研究中，我们对间充质干细胞中两种BMP - 2上调的miRNA，即miR - 24 - 1和miR - 31进行了表征，并展示了它们在控制细胞增殖和脂肪生成方面的相反功能。此外，我们首次鉴定并表征了小鼠内含子miR - 23b27b24 - 1和基因间miR - 31基因。而且，我们发现，尽管pri - miR - 23b、pri - miR - 27b和pri - miR - 24 - 1紧密相邻，但它们各自独立转录，并且在用BMP - 2处理细胞时，它们具有独特的表达谱。
