Wirenfeldt Martin, Clare Ryan, Tung Spencer, Bottini Alexander, Mathern Gary W, Vinters Harry V
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA.
Neurobiol Dis. 2009 Jun;34(3):432-40. doi: 10.1016/j.nbd.2009.02.015. Epub 2009 Mar 10.
Microgliosis is prominent in Rasmussen's encephalitis (RE), a disease with severe seizure activity. However, it is unclear if microglial activation is similar with different histopathologic substrates. Iba1-immunolabelled microglial activation was assessed in neocortex from pediatric epilepsy surgery patients with RE (n=8), cortical dysplasia (CD; n=6) and tuberous sclerosis complex (TSC; n=6). Microglial reactivity was increased, in severely affected RE areas (29% labeling) compared with minimally affected areas of RE cases (15%) and cases of TSC (14%) and CD (12%). There was no qualitative association of Iba1 immunolabelling with the presence of CD8(+) cytotoxic T-cells and no statistical association with clinical epilepsy variables, such as seizure duration or frequency. Iba1 appears to be an excellent marker for detecting extensive microglial activation in patients with RE. In addition, this study supports the notion that Iba1-labeled microglial activation is increased in patients with active RE, compared with cases of CD and TSC.
小胶质细胞增生在拉斯穆森脑炎（RE）中很突出，这是一种具有严重癫痫活动的疾病。然而，尚不清楚不同组织病理学底物的小胶质细胞激活是否相似。对患有RE（n = 8）、皮质发育异常（CD；n = 6）和结节性硬化症（TSC；n = 6）的小儿癫痫手术患者的新皮层进行了Iba1免疫标记的小胶质细胞激活评估。与RE病例的轻度受累区域（15%标记）、TSC病例（14%）和CD病例（12%）相比，严重受累的RE区域（29%标记）的小胶质细胞反应性增加。Iba1免疫标记与CD8(+)细胞毒性T细胞的存在没有定性关联，与癫痫持续时间或频率等临床癫痫变量也没有统计学关联。Iba1似乎是检测RE患者广泛小胶质细胞激活的优秀标志物。此外，本研究支持以下观点，即与CD和TSC病例相比，活动性RE患者中Iba1标记的小胶质细胞激活增加。
