Hadjigeorgiou M, Papachrysostomou Ch, Theodorou Z, Kanari P, Constantinou S
State General Laboratory of Cyprus, Veterinary Drug Residues Lab, Kimonos 44, 1451 Nicosia, Cyprus.
Anal Chim Acta. 2009 Apr 1;637(1-2):220-4. doi: 10.1016/j.aca.2009.01.001. Epub 2009 Jan 9.
Within the EU the use of dapsone (4,4-diaminodiphenylsulfone) is prohibited in food-producing animals and consequently it's included in the Annex IV of the Directive 90/2377/EC. A quantitative confirmatory method has been developed and validated according to the criteria defined in the Commission Decision 2002/657/EC, for the determination of dapsone in meat and milk. Samples, after homogenization in alkaline conditions and organic solvent extraction, were purified on silica gel solid phase extraction cartridges. The eluate was evaporated and redissolved in mobile phase and was analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) in positive electrospray ionisation (ESI) using deuterium labelled Sulphadimidine-d7 as internal standard. The calculated value for, decision limit, CCalpha is 0.12 microgkg(-1), and the detection capability; CCbeta value is 0.16 microgkg(-1).
在欧盟范围内，食品生产动物禁用氨苯砜（4,4-二氨基二苯砜），因此它被列入90/2377/EC号指令的附件IV中。已根据委员会第2002/657/EC号决定中规定的标准，开发并验证了一种用于测定肉类和牛奶中氨苯砜的定量确证方法。样品在碱性条件下匀浆并经有机溶剂萃取后，在硅胶固相萃取柱上进行净化。洗脱液经蒸发后再溶解于流动相中，采用液相色谱串联质谱法（LC-MS/MS），以氘代磺胺二甲嘧啶-d7作为内标，在正电喷雾电离（ESI）模式下进行分析。计算得出的决策限CCα值为0.12微克/千克，检测能力CCβ值为0.16微克/千克。
