Kong Wei-Jun, Zhao Yan-Ling, Xiao Xiao-He, Li Zu-Lun, Ren Yong-Shen
PLA Institute of Chinese Materia Medica, 302 Hospital of People's Liberation Army, Beijing, PR China.
J Hazard Mater. 2009 Sep 15;168(2-3):609-13. doi: 10.1016/j.jhazmat.2009.02.071. Epub 2009 Feb 23.
Using a thermal activity monitor (TAM) air isothermal microcalorimeter with ampoule mode, the thermo-genic curves of the metabolism of Tetrahymena thermophila BF(5) growth at 28 degrees C were obtained and the action of palmatine on it was investigated. Meanwhile, the biomass change during the process of T. thermophila BF(5) growth coexisted with palmatine was studied by a haemacytometer. The results showed that a low concentration (50 microg/mL) of palmatine began to inhibit the growth of T. thermophila BF(5), and when the concentration of palmatine reached 600 microg/mL, T. thermophila BF(5) could not grow at all. The relationship between the growth rate constant (k) and the concentration c was almost linear with the correlation coefficient of 0.9957, showing the strong toxic action of palmatine on T. thermophila BF(5) growth. The biomass during T. thermophila BF(5) growth decreased obviously by the addition of palmatine at different concentrations. The investigation of biomass agreed well with the results obtained by means of microcalorimetry.
采用具有安瓿模式的热活性监测仪（TAM）空气等温微量热计，获得嗜热四膜虫BF（5）在28℃生长代谢的产热曲线，并研究了巴马汀对其的作用。同时，用血细胞计数板研究了嗜热四膜虫BF（5）与巴马汀共存生长过程中的生物量变化。结果表明，低浓度（50μg/mL）的巴马汀开始抑制嗜热四膜虫BF（5）的生长，当巴马汀浓度达到600μg/mL时，嗜热四膜虫BF（5）完全不能生长。生长速率常数（k）与浓度c之间的关系几乎呈线性，相关系数为0.9957，表明巴马汀对嗜热四膜虫BF（5）生长具有较强的毒性作用。添加不同浓度的巴马汀后，嗜热四膜虫BF（5）生长过程中的生物量明显下降。生物量的研究结果与微量热法所得结果吻合良好。
