Pakotiprapha Danaya, Liu Yi, Verdine Gregory L, Jeruzalmi David
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
J Biol Chem. 2009 May 8;284(19):12837-44. doi: 10.1074/jbc.M900571200. Epub 2009 Mar 13.
Nucleotide excision repair is distinguished from other DNA repair pathways by its ability to process a wide range of structurally unrelated DNA lesions. In bacteria, damage recognition is achieved by the UvrA.UvrB ensemble. Here, we report the structure of the complex between the interaction domains of UvrA and UvrB. These domains are necessary and sufficient for full-length UvrA and UvrB to associate and thereby form the DNA damage-sensing complex of bacterial nucleotide excision repair. The crystal structure and accompanying biochemical analyses suggest a model for the complete damage-sensing complex.
核苷酸切除修复与其他DNA修复途径的区别在于，它能够处理多种结构不相关的DNA损伤。在细菌中，损伤识别是由UvrA-UvrB复合体实现的。在此，我们报道了UvrA和UvrB相互作用结构域之间复合物的结构。这些结构域对于全长UvrA和UvrB的结合是必要且充分的，从而形成细菌核苷酸切除修复的DNA损伤传感复合物。晶体结构及相关生化分析提出了一个完整损伤传感复合物的模型。
