在巧克力琼脂中添加异维他命用于流感嗜血杆菌的分离。
Addition of isovitalex in chocolate agar for the isolation of Haemophilus influenzae.
作者信息
Saha S K, Baqui A H, Darmstadt G L, Islam M, Arifeen S E, Santosham M, Nagatake T, Black R E
机构信息
Department of Microbiology, Dhaka Shishu (Children) Hospital, Dhaka, Bangladesh Institute of Child Health, Dhaka, Bangladesh.
出版信息
Indian J Med Res. 2009 Jan;129(1):99-101.
BACKGROUND & OBJECTIVE: The reason for lack of data on burden of Haemophilus influenzae type b (Hib) in developing countries was mainly failure of detection of this fastidious organism in laboratories. Use of isovitalex (IVX) was suggested as an essential supplement for growing this organism. This study was carried out to investigate the impact of IVX supplementation to chocolate agar for detection of Hib.
METHODS
Chocolate agar with and without supplementation of IVX was prepared. Clinical samples as well as reference strains of Hib were simultaneously cultured on both the media.
RESULTS
H. influenzae isolates (N=194) were simultaneously grown on chocolate agar (CA) with and without isovitalex (IVX). Average colony size of H. influenzae on CA with IVX (CA-IVX) was larger only by 0.10 cm (range 0.05 to 0.16 cm) compared to CA alone. Addition of IVX to CA increased the cost of media by 2.1-fold.
INTERPRETATION & CONCLUSION: Isovitalex is not essential for the isolation and growth of H. influenzae almost halving the cost.
背景与目的
发展中国家缺乏b型流感嗜血杆菌（Hib）负担数据的主要原因是实验室难以检测出这种苛求菌。有人建议使用异维生素（IVX）作为培养该菌的必要补充剂。本研究旨在探讨在巧克力琼脂中添加IVX对检测Hib的影响。
方法
制备添加和不添加IVX的巧克力琼脂。临床样本以及Hib参考菌株同时在两种培养基上培养。
结果
194株流感嗜血杆菌分离株在添加和不添加异维生素（IVX）的巧克力琼脂（CA）上同时生长。与单独的CA相比，添加IVX的CA（CA-IVX）上流感嗜血杆菌的平均菌落大小仅大0.10厘米（范围为0.05至0.16厘米）。向CA中添加IVX使培养基成本增加了2.1倍。
解读与结论
异维生素对于流感嗜血杆菌的分离和生长并非必不可少，成本几乎减半。
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