Gandhimathi R, Seghal Kiran G, Hema T A, Selvin Joseph, Rajeetha Raviji T, Shanmughapriya S
Department of Microbiology, Bharathidasan University, Tiruchirappalli 620024, India.
Bioprocess Biosyst Eng. 2009 Oct;32(6):825-35. doi: 10.1007/s00449-009-0309-x. Epub 2009 Mar 14.
A sponge-associated marine actinomycetes Nocardiopsis alba MSA10 was screened and evaluated for the production of biosurfactant. Biosurfactant production was confirmed by conventional screening methods including hemolytic activity, drop collapsing test, oil displacement method, lipase production and emulsification index. The active compound was extracted with three solvents including ethyl acetate, diethyl ether and dichloromethane. The diethyl ether extract was fractionated by TLC and semi-preparative HPLC to isolate the pure compound. In TLC, a single discrete spot was obtained with the R (f) 0.60 and it was extrapolated as valine. Based on the chemical characterization, the active compound was partially confirmed as lipopeptide. The optimum production was attained at pH 7, temperature 30 degrees C, and 1% salinity with glucose and peptone supplementation as carbon and nitrogen sources, respectively. Considering the biosurfactant production potential of N. alba, the strain could be developed for large-scale production of lipopeptide biosurfactant.
筛选并评估了一种与海绵相关的海洋放线菌——白色诺卡氏菌MSA10产生生物表面活性剂的能力。通过包括溶血活性、液滴塌陷试验、油置换法、脂肪酶产生和乳化指数在内的传统筛选方法确认了生物表面活性剂的产生。用乙酸乙酯、二乙醚和二氯甲烷三种溶剂提取活性化合物。二乙醚提取物通过薄层层析（TLC）和半制备高效液相色谱法进行分离以分离出纯化合物。在薄层层析中，获得了一个R（f）值为0.60的单一离散斑点，并推断其为缬氨酸。基于化学表征，活性化合物被部分确认为脂肽。在pH值为7、温度为30℃、盐度为1%的条件下，分别添加葡萄糖和蛋白胨作为碳源和氮源时，产量达到最佳。考虑到白色诺卡氏菌产生生物表面活性剂的潜力，该菌株可用于大规模生产脂肽生物表面活性剂。
