Jiang Zi-wei, Mo Hong-mei, Wang Lei, Cheng Yu-li, Wang Qi-hong, Lei Jia-hui, Liu Wen-qi, Li Yong-long
Department of Pathogenic Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2008 Dec 30;26(6):428-31, 437.
To investigate the effect of antigens of different stage Schistosoma japonicum on airway inflammation in a murine model of asthma.
48 female BALB/c mice were randomly divided into eight groups. Mice in group A were given normal saline of equal volume as control. Group B was asthma model which was established by intraperitoneal and intranasal challenge with OVA. Mice in groups C, D and E were immunized with soluble egg antigen (SEA), soluble male worm antigen (SWA), and schistosomulum antigen (SSA) respectively 4 times in a week interval, followed by OVA sensitization as in group B 1 week after the final immunization. Mice in groups F, G, and H were immunized with SEA, SWA, and SSA respectively but sensitized and challenged with saline instead of OVA. 48 hours after asthma was induced, the mice were sacrificed. Leukocytes and eosinophils were counted in bronchoalveolar lavage fluid (BALF). The level of IL-5, IL-10 and IFN-gamma in BALF was detected. Pathologic changes in lung tissues were observed.
Inflammation cells, especially eosinophils, appeared in airways of mice in groups B, C, D and E, but with much less number in groups C, D and E. No inflammation cells were seen in airways of group A mice. The number of leukocytes, eosinophils and level of IL-5 in BALF of group B [(98.4 +/- 16.1) x 10(4)/ml, (17.6 +/- 4.3) x 10(4)/ml, (197.9 +/- 36.5) pg/ml respectively] were significantly higher than those of group A [(8.2 +/- 1.1) x 10(4)/ml, (0.02 +/- 0.01) x 10(4)/ ml, (12.3 +/- 7.4) pg/ml], however the levels of IL-10 and IFN-gamma were significantly lower than that of group A (P < 0.05). The number of leukocytes, especially eosinophils, in BALF of groups C, D and E was significantly lower than that of group B. The level of IL-5 in BALF of groups C, D and E was significantly reduced, while that of IL-10 and IFN-gamma in BALF of the 3 groups was significantly higher than group B (P < 0.05).
The immunization with S. japonicum antigens can effectively modulate the level of cytokines and inhibit the eosinophil infiltration and airway inflammation in asthmatic mice.
探讨日本血吸虫不同阶段抗原对哮喘小鼠模型气道炎症的影响。
48只雌性BALB/c小鼠随机分为8组。A组小鼠给予等体积生理盐水作为对照。B组为哮喘模型组，通过腹腔注射和滴鼻给予卵清蛋白（OVA）建立模型。C、D、E组小鼠分别以可溶性虫卵抗原（SEA）、可溶性雄虫抗原（SWA）和童虫抗原（SSA）每周免疫1次，共4次，末次免疫1周后同B组进行OVA致敏。F、G、H组小鼠分别用SEA、SWA和SSA免疫，但用生理盐水代替OVA进行致敏和激发。诱发哮喘48小时后处死小鼠，计数支气管肺泡灌洗液（BALF）中的白细胞和嗜酸性粒细胞，检测BALF中白细胞介素-5（IL-5）、白细胞介素-10（IL-10）和干扰素-γ（IFN-γ）水平，观察肺组织病理变化。
B、C、D、E组小鼠气道出现炎症细胞，尤其是嗜酸性粒细胞，但C、D、E组数量明显较少。A组小鼠气道未见炎症细胞。B组BALF中白细胞、嗜酸性粒细胞数量及IL-5水平[分别为(98.4±16.1)×10⁴/ml、(17.6±4.3)×10⁴/ml、(197.9±36.5)pg/ml]显著高于A组[(8.2±1.1)×10⁴/ml、(0.02±0.01)×10⁴/ml、(12.3±7.4)pg/ml]，而IL-10和IFN-γ水平显著低于A组（P<0.05）。C、D、E组BALF中白细胞尤其是嗜酸性粒细胞数量显著低于B组。C、D、E组BALF中IL-5水平显著降低，而3组BALF中IL-10和IFN-γ水平显著高于B组（P<0.05）。
日本血吸虫抗原免疫可有效调节细胞因子水平，抑制哮喘小鼠嗜酸性粒细胞浸润和气道炎症。
