Kavanagh Katherine R, Parham Kourosh, Schoem Scott R
Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Connecticut Health Center, Farmington, CT 06030, USA.
Arch Otolaryngol Head Neck Surg. 2009 Mar;135(3):238-41. doi: 10.1001/archoto.2008.559.
To test for ototoxicity after prolonged ototopical fluoroquinolone use in the middle ear space using a murine model.
Nonrandomized controlled trial.
Twelve CBA/J mice.
The mice received daily intratympanic injections of ciprofloxacin-dexamethasone otic suspension for 21 days. The contralateral ear received daily intratympanic injections of sterile isotonic sodium chloride solution (saline) as a control.
Click-evoked auditory brainstem response (ABR) thresholds were obtained before injection and 10 days and 3 months after injection.
Mean (SEM) preinjection ABR thresholds were 47.17 (2.74) dB peak equivalent sound pressure level (peSPL) in ciprofloxacin-dexamethasone-treated ears and 45.08 (1.56) dB peSPL in saline-treated ears (P = .38). Mean (SEM) postinjection ABR thresholds in ciprofloxacin-dexamethasone-treated ears were 44.25 (1.25) dB peSPL after 10 days and 43.00 (1.51) dB peSPL after 3 months. Mean (SEM) postinjection ABR thresholds in saline-treated ears were 48.00 (1.51) dB peSPL after 10 days and 45.92 (1.79) dB peSPL after 3 months. There were no significant differences in ABR thresholds for ciprofloxacin-dexamethasone-treated ears (P = .29, P = .10) or saline-treated ears (P = .07, P = .59).
Ciprofloxacin-dexamethasone suspension did not cause either immediate or delayed ototoxicity after 21 days of intratympanic administration in the CBA/J mouse model.
使用小鼠模型测试中耳腔内长期局部使用氟喹诺酮类药物后的耳毒性。
非随机对照试验。
12只CBA/J小鼠。
小鼠每天接受鼓室内注射环丙沙星 - 地塞米松耳用混悬液，持续21天。对侧耳每天接受鼓室内注射无菌等渗氯化钠溶液（生理盐水）作为对照。
在注射前、注射后10天和3个月时获取短声诱发的听觉脑干反应（ABR）阈值。
环丙沙星 - 地塞米松治疗组耳注射前ABR阈值的平均值（标准误）为47.17（2.74）dB峰值等效声压级（peSPL），生理盐水治疗组耳为45.08（1.56）dB peSPL（P = 0.38）。环丙沙星 - 地塞米松治疗组耳注射后10天的ABR阈值平均值（标准误）为44.25（1.25）dB peSPL，3个月后为43.00（1.51）dB peSPL。生理盐水治疗组耳注射后10天的ABR阈值平均值（标准误）为48.00（1.51）dB peSPL，3个月后为45.92（1.79）dB peSPL。环丙沙星 - 地塞米松治疗组耳的ABR阈值（P = 0.29，P = 0.10）或生理盐水治疗组耳的ABR阈值（P = 0.07，P = 0.59）均无显著差异。
在CBA/J小鼠模型中，鼓室内给药21天后，环丙沙星 - 地塞米松混悬液未引起即刻或延迟性耳毒性。
