Treves A J, Feldman M, Kaplan H S
J Natl Cancer Inst. 1977 May;58(5):1527-9. doi: 10.1093/jnci/58.5.1527.
Cytotoxic effector T-lymphocytes were produced in vitro by sensitization of spleen cells on monolayers of syngeneic macrophages that had been fed with radiation leukemia virus-containing cell extracts or with supernatants of virus-producing cell cultures. The sensitized lymphocytes were cytotoxic to cell lines that expressed viral antigens. Secondary mouse embryo fibroblasts were little affected. Sensitization via macrophages appeared to be a useful system for identification of viral antigens on surfaces of various target cells, as well as for tests of the protective effect of such lymphocytes against tumor growth in vivo.
通过用含有辐射白血病病毒的细胞提取物或病毒产生细胞培养物的上清液喂养的同基因巨噬细胞单层对脾细胞进行致敏，在体外产生细胞毒性效应T淋巴细胞。致敏淋巴细胞对表达病毒抗原的细胞系具有细胞毒性。二次传代的小鼠胚胎成纤维细胞几乎没有受到影响。通过巨噬细胞致敏似乎是一种有用的系统，可用于鉴定各种靶细胞表面的病毒抗原，以及测试此类淋巴细胞对体内肿瘤生长的保护作用。
