庆大霉素与抗炎剂双氯芬酸对单核细胞增生李斯特菌的体外协同作用。

In vitro synergistic effect of gentamicin with the anti-inflammatory agent diclofenac against Listeria monocytogenes.

作者信息

Dutta N K, Mazumdar K, Park J-H

机构信息

College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, Korea.

出版信息

Lett Appl Microbiol. 2009 Jun;48(6):783-5. doi: 10.1111/j.1472-765X.2009.02588.x. Epub 2009 Mar 7.

DOI:10.1111/j.1472-765X.2009.02588.x

PMID:19291208

Abstract

AIMS

A total of nine Listeria monocytogenes strains (seven serotypes) were studied to ascertain whether the non-steroidal anti-inflammatory drug diclofenac (Dc) used in combination with the conventional antilisterial antibiotic gentamicin (Gm) or ampicillin (Am) synergistically augments the efficacy of the antibiotic in vitro.

METHODS AND RESULTS

The effect of combination was evaluated by the checkerboard method to obtain a fractional inhibitory concentration (FIC) index followed by kill curves. Dc was synergistic with Gm (FIC 0.37) and there was indifference with Am (FIC 1) against L. monocytogenes ATCC 51774. The magnitude of the differences between killing by a single agent and the combination observed at 24 h was significant (P < 0.05) for Dc plus Gm but not Dc plus Am.

CONCLUSIONS

Thus, the ability of extended antibiotic therapy may be improved with the help of this synergistic drug pair in listeriosis.

SIGNIFICANCE AND IMPACT OF THE STUDY

Such findings may indicate parallel administration of anti-inflammatory and anti listeriosis drugs.

摘要

目的

共研究了9株单核细胞增生李斯特菌菌株（7种血清型），以确定非甾体抗炎药双氯芬酸（Dc）与传统抗李斯特菌抗生素庆大霉素（Gm）或氨苄西林（Am）联合使用是否能在体外协同增强抗生素的疗效。

方法与结果

采用棋盘法评估联合用药的效果，以获得分数抑菌浓度（FIC）指数，随后绘制杀菌曲线。Dc与Gm联合使用具有协同作用（FIC为0.37），而Dc与Am联合使用对单核细胞增生李斯特菌ATCC 51774无协同或拮抗作用（FIC为1）。在24小时观察到的单药杀菌与联合用药杀菌之间的差异幅度，Dc加Gm具有显著性（P < 0.05），而Dc加Am则无显著性。