Zhu Qiyong
Department of Chemistry, Huainan Normal University, Huainan 232001, People's Republic of China.
Luminescence. 2009 Jul-Aug;24(4):250-4. doi: 10.1002/bio.1108.
Based on the inhibition effect of rutin on the luminol-hydrogen peroxide chemiluminescence (CL) system catalyzed by tetrasulfonated colbalt phthalocyanine (CoTSPc), a sensitive flow-injection CL method has been developed for the determination of rutin. The CL reaction mechanism was carefully investigated by examining CL emission spectra, UV-visible spectra and variation of reaction conditions. It was found that there existed a linear relationship between CL intensity and the concentration of rutin in the range of 8.0 x 10(-9) to 1.0 x 10(-6) mol L(-1), and the detection limit is 3.8 x 10(-9) mol L(-1). This proposed method is sensitive, convenient and simple, and has been applied to the determination of rutin in commercial rutin tablets with satisfactory results.
基于芦丁对四磺酸钴酞菁（CoTSPc）催化的鲁米诺-过氧化氢化学发光（CL）体系的抑制作用，建立了一种灵敏的流动注射CL法测定芦丁。通过考察CL发射光谱、紫外可见光谱以及反应条件的变化，对CL反应机理进行了深入研究。结果表明，在8.0×10^(-9)至1.0×10^(-6) mol L^(-1)范围内，CL强度与芦丁浓度呈线性关系，检出限为3.8×10^(-9) mol L^(-1)。该方法灵敏、简便，已应用于市售芦丁片中芦丁的测定，结果令人满意。
