Samadi-Maybodi Abdolraouf, Akhoondi Reza
Analytical Division, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
Luminescence. 2015 Sep;30(6):775-9. doi: 10.1002/bio.2819. Epub 2014 Nov 26.
N-Acetyl-L-cysteine (NAC) can inhibit the luminol-H2 O2 , reaction, which is catalyzed by silver nanoparticles. Based on this phenomenon a new method was developed for NAC determination. Under optimum conditions, a linear relationship between chemiluminescence intensity and NAC concentration was found in the range 0.034-0.98 µg/mL. The detection limit was 0.010 µg/mL (S/N =3), and the relative standard deviation (RSD) was <5% for 0.480 µg/mL NAC (n =5). This simple, sensitive and inexpensive method has been applied to measure the concentration of NAC in pharmaceutical tablets.
N-乙酰-L-半胱氨酸（NAC）能够抑制由银纳米颗粒催化的鲁米诺-H₂O₂反应。基于这一现象，开发了一种测定NAC的新方法。在最佳条件下，化学发光强度与NAC浓度在0.034 - 0.98 µg/mL范围内呈线性关系。检测限为0.010 µg/mL（S/N =3），对于0.480 µg/mL的NAC（n =5），相对标准偏差（RSD）<5%。这种简单、灵敏且廉价的方法已应用于测定药片中NAC的浓度。
