Jiang Zhiliang, Liang Yueyuan, Huang Guoxia, Wei Xiaoling, Liang Aihui, Zhong Fuxin
Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, School of Environment and Resources, Guangxi Normal University, Guilin 541004, People's Republic of China.
Luminescence. 2009 May-Jun;24(3):144-9. doi: 10.1002/bio.1079.
A highly sensitive and selective resonance scattering spectral assay was proposed for the determination of horseradish peroxidase (HRP), based on its catalytic effect on the H2O2 oxidation of KI to form I3(-). The I3(-) combined respectively with rhodamine (Rh) dye such as rhodamine S (RhS), rhodamine 6G (Rh6G), rhodamine B (RhB) and butyl-rhodamine B (b-RhB), to form association particles (Rh-I3(n). The four Rh systems all exhibit a stronger resonance scattering (RS) peak at 424 nm. For the RhS, Rh6G, RhB and b-RhB systems, HRP concentration in the range of 3.2 x 10(-12) to 4.8 x 10(-9), 2 x 10(-11) to 3.2 x 10(-9), 1.6 x 10(-11) to 3.2 x 10(-9) and 1.6 x 10(-11) to 4 x 10(-9) g/mL was linear to its RS intensity at 424 nm, with a detection limit of 2.2 x 10(-12), 2.5 x 10(-12), 4.4 x 10(-12) and 2.6 x 10(-12 )g/mL, respectively. This RhS system was most sensitive and stable, and was applied for the determination of HRP in the hepatitis B surface antibody labeling HRP and water samples, with satisfactory results.
基于辣根过氧化物酶（HRP）对H2O2氧化KI形成I3(-)的催化作用，提出了一种高灵敏度和高选择性的共振散射光谱分析法用于测定HRP。I3(-)分别与若丹明（Rh）染料如若丹明S（RhS）、若丹明6G（Rh6G）、若丹明B（RhB）和丁基若丹明B（b-RhB）结合，形成缔合粒子（Rh-I3(n)）。这四种Rh体系在424 nm处均呈现出较强的共振散射（RS）峰。对于RhS、Rh6G、RhB和b-RhB体系，HRP浓度在3.2×10(-12)至4.8×10(-9)、2×10(-11)至3.2×10(-9)、1.6×10(-11)至3.2×10(-9)和1.6×10(-11)至4×10(-9) g/mL范围内，其在424 nm处的RS强度呈线性关系，检测限分别为2.2×10(-12)、2.5×10(-12)、4.4×10(-12)和2.6×10(-12 )g/mL。该RhS体系最为灵敏和稳定，已应用于乙肝表面抗体标记HRP和水样中HRP的测定，结果令人满意。
