Qiu Yi-Hui, Zhang Yan, Hua Chun-Zhen, Zhang Zhu-Xian, Li Jian-Ping
Clinical Medical School of Hangzhou Normal University, Hangzhou 310018, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2009 Mar;11(3):217-20.
To investigate the serotypes distribution and ampicillin resistance of Haemophilus influenzae isolates from children with respiratory infection in Hangzhou.
Haemophilus influenzae strains were identified with V factor and X factor tests. Serotypes were determined with the slide agglutination method. Nitrocefin test was used to detect beta-lactamase. The sensitivities of ampicillin to Haemophilus influenzae were determined with the Kirby-Bauer diffusion method and the E-test method.
One hundred and fifty-two Haemophilus influenzae isolates, 108 from boys and 44 from girls, were identified between December 2006 and July 2007. Of the 152 isolates, 148 (97.4%) were untypable, only 4 (2.6%) were typable, including type a, type d, type e and type f (n=1 each type). Haemophilus influenzae type b and c strain was not found. Thirty-four isolates (22.4%) were beta-lactamase-positive. One hundred and thirteen isolates (74.3%) were susceptible to ampicillin, while 34 isolates (22.4%) were resistant to ampicillin.
Untypable strains were the most common in Haemophilus influenzae isolates from children with respiratory infection in Hangzhou. The isolates of Haemophilus influenzae kept susceptibity to ampicillin to a certain extent.
调查杭州地区呼吸道感染患儿流感嗜血杆菌分离株的血清型分布及对氨苄西林的耐药情况。
采用V因子和X因子试验鉴定流感嗜血杆菌菌株。用玻片凝集法确定血清型。用硝噻吩试验检测β-内酰胺酶。采用纸片扩散法和E试验法测定氨苄西林对流感嗜血杆菌的敏感性。
2006年12月至2007年7月间共鉴定出152株流感嗜血杆菌分离株，其中男童108株，女童44株。在这152株分离株中，148株（97.4%）无法分型，仅4株（2.6%）可分型，包括a型、d型、e型和f型（各型1株）。未发现b型和c型流感嗜血杆菌菌株。34株（22.4%）分离株β-内酰胺酶阳性。113株（74.3%）分离株对氨苄西林敏感，34株（22.4%）对氨苄西林耐药。
在杭州地区呼吸道感染患儿的流感嗜血杆菌分离株中，无法分型的菌株最为常见。流感嗜血杆菌分离株对氨苄西林仍有一定程度的敏感性。
