Chitwood D J, Hutzell P A, Lusby W R
J Nematol. 1985 Jan;17(1):64-8.
Sterols from free sterol and steryl ester fractions from Heterodera zeae and from total lipids of Zea mays roots were analyzed by gas-liquid chromatography (GLC) and by GLC-mass spectrometry. The major free sterols of H. zeae were 24-ethylcholesterol (54.4% of total free sterol), 24-ethylcholesta-5,22-dien-3beta-ol (13.3%), 24-methylcholesterol (12.5%), and cholesterol (7.2%). The same four sterols comprised 34.6%, 7.2%, 30.3%, and 18.6%, respectively, of the esterified sterols of H. zeae. Corn root sterols included 46.6% 24-ethylcholesta-5,22-dien-3beta-ol, 16.7% methylcholesterol, 16.4% cycloartenol, 12.7% 24-ethylcholesterol, and 0.5% cholesterol. The sterol 24-composition of H. zeae differed greatly from that of the only other cyst nematode previously investigated, Globodera solanacearum.
利用气液色谱法(GLC)和GLC-质谱分析法,对玉米异皮线虫游离甾醇和甾醇酯组分中的甾醇以及玉米根总脂中的甾醇进行了分析。玉米异皮线虫主要的游离甾醇为24-乙基胆固醇(占游离甾醇总量的54.4%)、24-乙基胆甾-5,22-二烯-3β-醇(13.3%)、24-甲基胆固醇(12.5%)和胆固醇(7.2%)。同样这四种甾醇在玉米异皮线虫酯化甾醇中分别占34.6%、7.2%、30.3%和18.6%。玉米根甾醇包括46.6%的24-乙基胆甾-5,22-二烯-3β-醇、16.7%的甲基胆固醇、16.4%的环阿屯醇、12.7%的24-乙基胆固醇和0.5%的胆固醇。玉米异皮线虫的甾醇24-组成与之前研究过的另一种孢囊线虫——茄科球孢囊线虫有很大差异。