Kuksis A, Myher J J, Marai L, Little J A, McArthur R G, Roncari D A
Lipids. 1986 Jun;21(6):371-7. doi: 10.1007/BF02534930.
The bulk of the plasma plant sterol in phytosterolemia occurs in the esterified form and is carried mostly in the low and high density lipoproteins. We have determined the fatty acid composition of the individual plasma steryl esters from a newly discovered subject with phytosterolemia and xanthomatosis. For this purpose the intact steryl esters were subject to high temperature gas liquid chromatography (GLC) on a polar capillary column, which separated the major esters on the basis of molecular weight and degree of unsaturation of the fatty acids. The saturated and unsaturated sterols esterified to saturated, monoenic, dienoic and tetraenoic fatty acids were identified by GLC analysis of the sterol moieties of the corresponding AgNO3-TLC fractions of the steryl esters. The GLC results were confirmed by reversed phase high performance liquid chromatography combined with mass spectrometry via direct liquid inlet interface. It was found that, in general, each fatty acid was esterified to the same complement of sterols, and that the esterified sterols possessed a composition comparable to that of the free plasma sterols, which was comprised of about 75% cholesterol, 6% campesterol, 4% 22,23-dihydrobrassicasterol and 15% beta-sitosterol. The fatty acid composition of the steryl esters differed from that of the 2-position of the plasma phosphatidylcholines, which contained significantly less palmitic and oleic and more linoleic acid. On the basis of these results and a review of the literature it is suggested that the plasma cholesteryl and plant steryl esters in phytosterolemia originate from both synthesis in plasma via the lecithin-cholesterol acyltransferase and synthesis in tissues via the acylCoA-cholesterol acyltransferase.
植物甾醇血症中大部分血浆植物甾醇以酯化形式存在，主要载于低密度和高密度脂蛋白中。我们已测定了一名新发现的患有植物甾醇血症和黄瘤病患者个体血浆甾醇酯的脂肪酸组成。为此，完整的甾醇酯在极性毛细管柱上进行高温气相液相色谱（GLC）分析，该柱根据脂肪酸的分子量和不饱和度分离主要酯类。通过对甾醇酯相应硝酸银 - 薄层色谱（TLC）馏分的甾醇部分进行GLC分析，鉴定了酯化到饱和、单烯、二烯和四烯脂肪酸的饱和与不饱和甾醇。GLC结果通过反相高效液相色谱结合直接液体进样接口的质谱分析得到证实。结果发现，一般来说，每种脂肪酸酯化到相同的甾醇组合，且酯化甾醇的组成与游离血浆甾醇相当，游离血浆甾醇约由75%胆固醇、6%菜油甾醇、4% 22,23 - 二氢油菜甾醇和15%β - 谷甾醇组成。甾醇酯的脂肪酸组成与血浆磷脂酰胆碱2位的脂肪酸组成不同，后者含有的棕榈酸和油酸明显较少，亚油酸较多。基于这些结果并结合文献综述，提示植物甾醇血症中的血浆胆固醇酯和植物甾醇酯既来源于血浆中通过卵磷脂 - 胆固醇酰基转移酶的合成，也来源于组织中通过酰基辅酶A - 胆固醇酰基转移酶的合成。
