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从土壤中提取甜菜孢囊线虫的孢囊和卵并评估提取效率

Extraction of Cysts and Eggs of Heterodera schachtii from Soil with an Assessment of Extraction Efficiency.

作者信息

Caswell E P, Thomason I J, McKinney H E

出版信息

J Nematol. 1985 Jul;17(3):337-40.

PMID:19294103
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2618451/
Abstract

Techniques are described for the extraction and enumeration of cysts and contained eggs from soil samples. The average recovery of cysts from seeded soil samples of differing soil texture was 82.7 +/- 2.1%. Recovery from sandy clay soil samples seeded at 1 cyst/100 g soil was 63.4 +/- 5.5%; at 4.2 cysts/100 g soil recovery was 89.6 +/- 1.8%. Recovery of cysts from naturally infested clay soil was 88.3 +/- 2.05%. Egg extraction efficiency for seeded samples was calculated as 78%, and for naturally infested soil was estimated as 83%.

摘要

本文描述了从土壤样本中提取和计数囊肿及其中所含虫卵的技术。不同土壤质地的接种土壤样本中囊肿的平均回收率为82.7±2.1%。在每100克土壤接种1个囊肿的砂质粘土样本中,回收率为63.4±5.5%;在每100克土壤接种4.2个囊肿时,回收率为89.6±1.8%。天然感染的粘土样本中囊肿的回收率为88.3±2.05%。接种样本的虫卵提取效率计算为78%,天然感染土壤的虫卵提取效率估计为83%。

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