Wang QiuYue, Li JianHua, Zhang XiChen, Liu ChengWu, Cao LiLi, Ren KeYan, Gong PengTao, Cai YaNan
College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062, China.
Sci China C Life Sci. 2009 Mar;52(3):278-83. doi: 10.1007/s11427-009-0008-5. Epub 2009 Mar 18.
Chicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry industry. Enhanced green fluorescent protein (EGFP) tagged recombinant Bacille Calmette-Guerin (rBCG), as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study. Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response. The colonization of rBCG in liver, spleen, lung, kidney and caecum was observed by laser confocal microscopy. Real-time quantitative RT-PCR showed a rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization. These results have significant implications for the development of rBCG vaccines against avian coccidiosis.
鸡球虫病是一种对家禽业造成巨大经济负担的主要寄生虫病。本研究构建了增强型绿色荧光蛋白（EGFP）标记的重组卡介苗（rBCG），其作为与球虫菱形抗原的融合蛋白，用于在鸡体内追踪rBCG。用一剂rBCG pMV361-Rho/EGFP免疫鸡可诱导体液免疫反应。通过激光共聚焦显微镜观察到rBCG在肝脏、脾脏、肺、肾脏和盲肠中的定植情况。实时定量RT-PCR显示，免疫后第7天菱形蛋白表达水平升高，第14天达到峰值，第28天消失。这些结果对开发抗禽球虫病的rBCG疫苗具有重要意义。
