Fraser Iain D C, Germain Ronald N
Molecular and Cell Biology Group, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Nat Immunol. 2009 Apr;10(4):327-31. doi: 10.1038/ni.1711. Epub 2009 Mar 19.
Recent studies in hematopoietic cells have led to a growing appreciation of the diverse modes of molecular and functional cross-talk between canonical signaling pathways. However, these intersections represent only the tip of the iceberg. Emerging global analytical methods are providing an even richer and more complete picture of the many components that measurably interact in a network manner to produce cellular responses. Here we highlight the pieces in this Focus, emphasize the limitations of the present canonical pathway paradigm, and discuss the value of a systems biology approach using more global, quantitative experimental design and data analysis strategies. Lastly, we urge caution about overly facile interpretation of genome- and proteome-level studies.
最近在造血细胞中的研究使人们越来越认识到经典信号通路之间分子和功能相互作用的多种模式。然而，这些交叉点只是冰山一角。新兴的全局分析方法正在提供一幅更丰富、更完整的图景，展示了许多以网络方式可测量地相互作用以产生细胞反应的成分。在这里，我们突出了本专题中的要点，强调了当前经典通路范式的局限性，并讨论了使用更全局、定量的实验设计和数据分析策略的系统生物学方法的价值。最后，我们敦促对基因组和蛋白质组水平研究的过度简单化解释保持谨慎。
