Vujanovic V, Vidovic S, Fernandez M R, Daida P, Korber D
Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK-S7N-5A8, Canada.
Can J Microbiol. 2009 Feb;55(2):117-25. doi: 10.1139/w08-103.
A total of 91 isolates of Fusarium avenaceum were regrouped into 15 phenotypes and 10 vegetative compatibility groups showing specific one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1-D SDS-PAGE) protein profiles and less-specific internal transcribed spacer rDNA profiles. Each isolate possessed reproducible signature protein bands. Indeed, the unweighted pair group method with arithmetic averages clustering revealed that the protein profile of each group of isolates correlated with fungus virulence. The use of SDS-PAGE offers a simple and sensitive technique for routine differentiation between pathogenic and nonpathogenic isolates within unknown F. avenaceum populations. The discovery has significant implications for risk assessment of cereal yield to ensure food and feed safety. This low-cost approach has the potential to be optimized and extended to a broad spectrum of Fusarium head blight pathogens.
总共91株燕麦镰刀菌被重新划分为15种表型和10个营养亲和群，它们呈现出特定的一维十二烷基硫酸钠聚丙烯酰胺凝胶电泳（1-D SDS-PAGE）蛋白质谱和特异性较低的内转录间隔区rDNA谱。每个分离株都有可重复的特征蛋白条带。事实上，算术平均非加权配对组法聚类分析表明，每组分离株的蛋白质谱与真菌毒力相关。SDS-PAGE的应用为未知燕麦镰刀菌群体中致病和非致病分离株的常规鉴别提供了一种简单且灵敏的技术。这一发现对谷物产量的风险评估具有重要意义，以确保食品和饲料安全。这种低成本方法有可能得到优化并扩展到广泛的镰刀菌穗腐病病原体。
