Visentin I, Tamietti G, Valentino D, Portis E, Karlovsky P, Moretti A, Cardinale F
DiVaPRA, Plant Pathology, University of Turin, via L. da Vinci 44, 10095 Grugliasco (TO), Italy.
Mycol Res. 2009 Oct;113(Pt 10):1137-45. doi: 10.1016/j.mycres.2009.07.011. Epub 2009 Jul 23.
The maize pathogens Fusarium verticillioides (Fv) and Fusarium proliferatum (Fp) are morphologically very similar to one another, so Fp isolates have been often mistaken as Fusarium moniliforme (the former name of Fv). The only presently accepted morphological discriminator between these species is the presence/absence of polyphialides. Here, a collection of 100 Fusarium strains, isolated from infected maize kernels on plants grown in north-western Italy, were assigned as Fv or Fp on the basis of the presence/absence of polyphialides. This classification was tested on a subset of isolates by sexual crosses, ITS and calmodulin sequencing and AFLP profiling. An ITS-RFLP assay was extended to the full collection and to a number of Fv and Fp isolates of different geographical origin and hosts. The ITS region is proposed as taxonomically informative for distinguishing between Fp and Fv.
玉米病原菌轮枝镰孢菌（Fv）和层出镰孢菌（Fp）在形态上非常相似，因此Fp分离株常被误认为是串珠镰孢菌（Fv的曾用名）。目前这两个物种之间唯一被认可的形态鉴别特征是是否存在多瓶梗。在此，从意大利西北部种植的受感染玉米籽粒中分离出100株镰孢菌菌株，根据是否存在多瓶梗将其鉴定为Fv或Fp。通过有性杂交、ITS和钙调蛋白测序以及AFLP分析，对一部分分离株的这种分类进行了验证。一种ITS-RFLP分析方法被扩展应用于整个菌株集合以及一些来自不同地理来源和寄主的Fv和Fp分离株。ITS区域被认为在区分Fp和Fv方面具有分类学信息价值。
