Steele A E, Whitehand L
J Nematol. 1984 Apr;16(2):171-7.
Measurements of second-stage juveniles of Heterodera schachtii from California and The Netherlands and a race of H. trifolii from The Netherlands were obtained and compared to determine if these populations can be differentiated by morphometrics. Juvenile lengths of 10 specimens from each of 10 cysts of each population were measured. Dimensions of tail regions of 20 juveniles from individual cysts of H. schachtii (California) and a like number of juveniles of H. trifolii (The Netherlands) were also obtained. The mean lengths of juveniles of H. schachtii from California and The Netherlands were not significantly different, but similar measurements of H. schachtii and H. trifolii were different (P = 0.05). Mean dimensions of tail lengths, tail widths, tail hyaline lengths, and tail length/tail width were significantly greater for H. trifolii than for H. schachtii. Also, dimensions of eggs of H. trifolii were significantly greater than dimensions of H. schachtii eggs. The investigations established that H. schachtii can be readily differentiated from H. trifolii by morphometrics of eggs and juveniles, Minimum sample sizes required for specified confidence intervals for each criterion measured are provided.
对来自加利福尼亚州和荷兰的甜菜孢囊线虫二期幼虫以及来自荷兰的三叶草孢囊线虫一个生理小种进行了测量并比较，以确定这些群体是否可以通过形态测量学进行区分。测量了每个群体10个孢囊中各10个标本的幼虫长度。还获取了来自加利福尼亚州甜菜孢囊线虫单个孢囊的20条幼虫以及相同数量的荷兰三叶草孢囊线虫幼虫的尾部尺寸。来自加利福尼亚州和荷兰的甜菜孢囊线虫幼虫平均长度没有显著差异，但甜菜孢囊线虫和三叶草孢囊线虫的类似测量值不同（P = 0.05）。三叶草孢囊线虫的尾长、尾宽、尾透明区长和尾长/尾宽的平均尺寸显著大于甜菜孢囊线虫。此外，三叶草孢囊线虫卵的尺寸显著大于甜菜孢囊线虫卵的尺寸。这些研究表明，通过卵和幼虫的形态测量学可以很容易地将甜菜孢囊线虫与三叶草孢囊线虫区分开来，并提供了每个测量标准在特定置信区间所需的最小样本量。
