Laboratório of Molecular Genetics Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB/UNL), Oeiras, Portugal.
Microb Drug Resist. 2009 Mar;15(1):19-26. doi: 10.1089/mdr.2009.0881.
This two-year study investigated the epidemiology of nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) among patients and healthcare workers (HCWs) in two wards with a high frequency of MRSA isolation, at Hospital Geral de Santo António (HGSA), Portugal. Three point-prevalence surveys per year were carried out. A case-control approach was used to identify potential risk factors associated with MRSA carriage among patients. Incidence rates and risk factors of MRSA carriage among HCWs who were negative at the baseline observation were estimated. Prevalence of MRSA carriage among 276 patients screened was 5.1%. Admission to HGSA or attendance to the Diabetic Foot Outpatient Unit (DFOU) of HGSA within the past 12 months, and previous MRSA isolation were significant risk factors for MRSA carriage. Among HCWs (n = 126), the prevalence of MRSA carriage was 4.8% and the incidence rate was 61/1000 person-years. Nurses and nurse aids were the HCW categories with the highest risk of becoming colonized with MRSA over time (p = 0.01). One HCW chronically colonized was detected. Molecular typing revealed a clonal identity for isolates recovered from patients and HCWs of the same wards, with 88.6% of isolates belonging to the EMRSA-15 (ST22-MRSA-IV) clone.
这项为期两年的研究调查了葡萄牙圣安东尼综合医院（HGSA）两个高耐甲氧西林金黄色葡萄球菌（MRSA）分离频率病房的患者和医护人员（HCW）中鼻腔定植 MRSA 的流行病学情况。每年进行三次时点患病率调查。采用病例对照方法确定与患者 MRSA 定植相关的潜在危险因素。对基线观察时为阴性的 HCW 中 MRSA 定植的发生率和危险因素进行了估计。276 名筛查患者中，MRSA 定植率为 5.1%。过去 12 个月内入住 HGSA 或在 HGSA 糖尿病足门诊就诊，以及既往 MRSA 分离是 MRSA 定植的显著危险因素。在 126 名 HCW 中，MRSA 定植率为 4.8%，发病率为 61/1000 人年。随着时间的推移，护士和护士助理成为最容易被 MRSA 定植的 HCW 类别（p = 0.01）。发现了一名慢性定植的 HCW。分子分型显示从同一病房的患者和 HCW 中分离出的菌株具有克隆同一性，88.6%的分离株属于 EMRSA-15（ST22-MRSA-IV）克隆。
