通过高速逆流色谱法分离黄嘌呤亭，一种蚂蚁共生真菌的抑制剂。
Isolation of xanthyletin, an inhibitor of ants' symbiotic fungus, by high-speed counter-current chromatography.
作者信息
Cazal Cristiane de Melo, Domingues Vanessa de Cássia, Batalhão Jaqueline Raquel, Bueno Odair Corrêa, Filho Edson Rodrigues, da Silva Maria Fátima G Fernandes, Vieira Paulo Cezar, Fernandes João Batista
机构信息
Universidade Federal de São Carlos, Departamento de Química, SP, Brazil.
出版信息
J Chromatogr A. 2009 May 8;1216(19):4307-12. doi: 10.1016/j.chroma.2009.02.066. Epub 2009 Mar 3.
Xanthyletin, an inhibitor of symbiotic fungus (Leucoagaricus gongylophorus) of leaf-cutting ant (Atta sexdens rubropilosa), as well as suberosin, seselin and xanthoxyletin were isolated from Citrus sinensis grafted on Citrus limonia. A two-phase solvent system composed of hexane/ethanol/acetonitrile/water (10:8:1:1, v/v) was used for the high-speed counter-current chromatographic isolation of xanthyletin with high yield and over 99% purity as determined by liquid and gas chromatography with mass spectrometry detection. Identifications were performed by UV spectra, IR spectra, (1)H NMR and (13)C NMR.
从嫁接在黎檬上的甜橙中分离出了黄木亭（一种切叶蚁共生真菌（红褐举腹蚁）的抑制剂）以及软木栓质、邪蒿素和花椒亭。采用己烷/乙醇/乙腈/水（10:8:1:1，v/v）组成的两相溶剂系统，通过高速逆流色谱法高效分离出黄木亭，经液相色谱 - 质谱联用和气相色谱 - 质谱联用测定，其产率高且纯度超过99%。通过紫外光谱、红外光谱、¹H NMR和¹³C NMR进行鉴定。
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