Kára J, Cerná H, Dvorák M
Mol Cell Biochem. 1977 Feb 4;14(1-3):135-42. doi: 10.1007/BF01734177.
Experimental evidence for the presence and biosynthesis of subviral, leukemogenic particles in the isolated mitochondria of spleen cells of mice infected with Rauscher murine leukemia (RML) virus is presented. These subviral particles sediment at a density of 1.27-1.29 g/ml and induce splenomegaly and RML three weeks after i.v. or i.p. administration to white mice. Virosomes have been labelled with [32P]phosphate in the isolated mitochondria from RML spleen cells and high molecular weight (70S) [32P]RNA has been isolated from these subviral, leukemogenic particles. Rauscher virus group specific antigens were detected by immunodiffusion in the inner membrane and matrix fraction of the mitochondria of RML spleen cells. These results together with our earlier findings strongly suggest that mitochondria of the transformed cells participate in the biosynthesis of RNA tumor viruses. Possible mechanism of the penetration of viral genetic information of RNA tumor viruses into mitochondria of tumor cells in vivo is discussed.
本文提供了实验证据，证明感染劳舍尔鼠白血病（RML）病毒的小鼠脾脏细胞分离线粒体中存在亚病毒致白血病颗粒及其生物合成。这些亚病毒颗粒的沉降密度为1.27 - 1.29 g/ml，静脉或腹腔注射给小白鼠三周后会诱发脾肿大和RML。在来自RML脾脏细胞的分离线粒体中，病毒体已用[32P]磷酸盐标记，并且已从这些亚病毒致白血病颗粒中分离出高分子量（70S）[32P]RNA。通过免疫扩散在RML脾脏细胞线粒体的内膜和基质部分检测到劳舍尔病毒群特异性抗原。这些结果与我们早期的发现共同强烈表明，转化细胞的线粒体参与RNA肿瘤病毒的生物合成。文中还讨论了RNA肿瘤病毒的病毒遗传信息在体内侵入肿瘤细胞线粒体的可能机制。
