Blaschke R, Andres A C, Reid H H, Zürcher G, Friis R R, Ziemiecki A
Universität Bern, Abteilung für klinisch-experimentelle Forschung, Tiefenauspital, Schweiz.
Behring Inst Mitt. 1991 Jul(89):81-92.
Protein tyrosine kinases (PTKs) play a central role in cellular regulation by virtue of their participation in, and control of, signal transduction pathways; they act as a molecular interface between the cell's environment and intercellular metabolism. The mammary gland, unlike most organs, undergoes most of its morphogenesis in juvenile and adult life. The epithelium goes through hormonally controlled cycles of proliferation and regression, the fully differentiated state only being reached at the end of pregnancy. These features make the mammary gland an amenable tissue to study the involvement of PTKs in epithelial cell development and differentiation. We have used a PCR-based molecular cloning strategy to identify PTKs from murine mammary gland cells. Amongst 70 kinase clones characterized we found 3 PTKs previously undescribed in mouse, 4 known PTKs and 5 serine threonine kinases. Expression studies revealed differential tissue specificity and developmental regulation of the 3 previously undescribed PTKs. These results substantiate the view that PTKs are involved in the regulation of cellular differentiation.
蛋白质酪氨酸激酶（PTKs）通过参与并控制信号转导途径，在细胞调节中发挥核心作用；它们充当细胞环境与细胞间代谢之间的分子界面。与大多数器官不同，乳腺的大部分形态发生在幼年和成年期。上皮细胞经历激素控制的增殖和退化周期，只有在妊娠末期才达到完全分化状态。这些特征使乳腺成为研究PTKs参与上皮细胞发育和分化的理想组织。我们采用基于PCR的分子克隆策略，从鼠乳腺细胞中鉴定PTKs。在70个已鉴定的激酶克隆中，我们发现了3种先前在小鼠中未描述过的PTKs、4种已知的PTKs和5种丝氨酸苏氨酸激酶。表达研究揭示了这3种先前未描述过的PTKs的不同组织特异性和发育调控。这些结果证实了PTKs参与细胞分化调节的观点。
