通过荧光共振能量转移对IRGM四核苷酸启动子寡聚重复序列进行基因分型。

Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer.

作者信息

Intemann Christopher D, Thye Thorsten, Sievertsen Jürgen, Owusu-Dabo Ellis, Horstmann Rolf D, Meyer Christian G

机构信息

Bernhard Nocht Institute for Tropical Medicine, Department of Molecular Medicine, Hamburg, Germany.

出版信息

Biotechniques. 2009 Jan;46(1):58-60. doi: 10.2144/000113045.

DOI:10.2144/000113045

PMID:19301623

Abstract

Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using the LightTyper/LightCycler system. The protocol was evaluated and applied to the typing of a tetranucleotide promoter repeat of the human gene encoding the immunity-related GTPase family, M (IRGM) molecule in >2000 individuals from Ghana, West Africa.

摘要

荧光共振能量转移（FRET）基因分型技术已被广泛应用于单核苷酸多态性（SNP）和缺失的快速评估。本文介绍了一种利用LightTyper/LightCycler系统对短串联寡核苷酸重复序列进行分型的设计方案。该方案经过评估，并应用于对来自西非加纳的2000多名个体中编码免疫相关GTP酶家族M（IRGM）分子的人类基因四核苷酸启动子重复序列的分型。