Muniaraj M, Dinesh D S, Sinha P K, Das P, Bhattacharya S K
Centre for Research in Medical Entomology, Indian Council of Medical Research, No. 4, Sarojini Street, China Chokkikulam, Madurai, 625002, India.
J Commun Dis. 2008 Jun;40(2):133-8.
A simple dual culture agar plating technique has been developed and evaluated for its efficiency in determining the relationship of gut bacteria of sandfly with Leishmania donovani promastigotes. There are about twenty morphologically distinct bacterial colonies have been isolated from the gut homogenate of Phlebotomus argentipes. In dual culture method, each bacterial isolate was inoculated in one half of the plate and the promastigotes of Leishmania was inculcated in the other half by streaking. After incubation, the type of association was determined based on the presence or absence of promastigotes colonies. The reliability of this method was compared with broth dilution method in 96 well plate.
已开发并评估了一种简单的双培养琼脂平板接种技术，以确定白蛉肠道细菌与杜氏利什曼原虫前鞭毛体之间关系的效率。从银足白蛉的肠道匀浆中分离出了约20种形态不同的细菌菌落。在双培养法中，将每种细菌分离株接种在平板的一半，另一半通过划线接种杜氏利什曼原虫的前鞭毛体。培养后，根据前鞭毛体菌落的有无确定关联类型。将该方法的可靠性与96孔板中的肉汤稀释法进行了比较。
