Schlenzig Alexandra
Plant Health, Scottish Agricultural Science Agency, Edinburgh, UK.
Methods Mol Biol. 2009;508:161-9. doi: 10.1007/978-1-59745-062-1_13.
The following chapter describes a PCR method for the identification of the raspberry root rot pathogen Phytophthora fragariae var. rubi. Furthermore, a nested PCR suitable for the detection of the pathogen in infected raspberry roots and validated against the "Duncan bait test" (EPPO Bull 35:87-91, 2005) is explained. Protocols for different DNA extraction methods are given which can be transferred to other fungal pathogens.
以下章节描述了一种用于鉴定树莓根腐病病原菌草莓疫霉红树莓变种的聚合酶链式反应（PCR）方法。此外，还解释了一种适用于检测受感染树莓根中病原菌且已通过“邓肯诱饵试验”验证（《欧洲和地中海植物保护组织通报》35:87 - 91，2005年）的巢式PCR。文中给出了不同DNA提取方法的方案，这些方法可应用于其他真菌病原菌。
