百日咳毒素-生物素缀合物的合成与表征

Synthesis and characterization of a Pertussis toxin-biotin conjugate.

作者信息

Heerze L D, Clark C G, Chen Y, Smith R H, Armstrong G D

机构信息

Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.

出版信息

Biochem Biophys Res Commun. 1991 Sep 30;179(3):1464-9. doi: 10.1016/0006-291x(91)91737-w.

DOI:10.1016/0006-291x(91)91737-w

PMID:1930187

Abstract

We prepared a Pertussis toxin-biotin conjugate and found its biological properties to be similar to those of native Pertussis toxin with respect to the hemagglutination, Chinese hamster ovary cell, and lymphocyte proliferation assays. Direct binding to Chinese hamster ovary and Jurkat cells was observed using fluorescence microscopy. Pertussis toxin-biotin was also found to possess similar glycoconjugate binding specificities as those of 125I-labeled Pertussis toxin.

摘要

我们制备了百日咳毒素-生物素缀合物，并发现其在血凝、中国仓鼠卵巢细胞和淋巴细胞增殖试验方面的生物学特性与天然百日咳毒素相似。使用荧光显微镜观察到其与中国仓鼠卵巢细胞和Jurkat细胞的直接结合。还发现百日咳毒素-生物素具有与125I标记的百日咳毒素相似的糖缀合物结合特异性。