Senba M, Buziba N, Mori N, Wada A, Irie S, Toriyama K
Department of Pathology, Institute of Tropical Medicine, Nagasaki University, Japan.
Acta Virol. 2009;53(1):43-8. doi: 10.4149/av_2009_01_43.
Human papillomavirus (HPV) E6 and E7 gene products play a central role in the induction of benign proliferation and malignant transformation by interacting with several cellular regulatory proteins such as p53, p16(INK4a), and nuclear factor kappaB (NF-kappaB). In this study, HPV DNA was detected by in situ hybridization (ISH) and p53, p16(INK4a), and NF-kappaB by immunochemistry in 22 penile cancer cases in Kenya. HPV DNA was found in 68.2% of the cases. There was no difference in the p53- and p16(INK4a)-positivities in HPV DNA-positive and HPV DNA-negative cases. In HPV DNA-positive cases, the NF-kappaB positivity in the nucleus, cytoplasm, and nucleus and/or cytoplasm amounted to 73.3%, 93.3%, and 100%, respectively, while in HPV DNA-negative cases, a 28.7% NF-kappaB positivity of in the nucleus and/or cytoplasm was observed. It is concluded that NF-kappaB in penile cancer is expressed more frequently in the presence of HPV infection than in its absence.
人乳头瘤病毒（HPV）的E6和E7基因产物通过与多种细胞调节蛋白相互作用，如p53、p16（INK4a）和核因子κB（NF-κB），在良性增殖和恶性转化的诱导过程中发挥核心作用。在本研究中，对肯尼亚的22例阴茎癌病例采用原位杂交（ISH）检测HPV DNA，采用免疫化学方法检测p53、p16（INK4a）和NF-κB。68.2%的病例检测到HPV DNA。HPV DNA阳性和阴性病例的p53和p16（INK4a）阳性率没有差异。在HPV DNA阳性病例中，细胞核、细胞质以及细胞核和/或细胞质中的NF-κB阳性率分别为73.3%、93.3%和100%，而在HPV DNA阴性病例中，细胞核和/或细胞质中NF-κB阳性率为28.7%。得出的结论是，与未感染HPV相比，阴茎癌中NF-κB在HPV感染时表达更频繁。
