Yost R W, Grauvickel S J, Cantwell R, Bomalaski J S, Hudson A P
Research Service, Department of Veterans Affairs Medical Center, Philadelphia, PA 19104.
Biochem Int. 1991 May;24(2):199-208.
We demonstrate that both phospholipase A1 and phospholipase A2 are associated with isolated yeast mitochondria (Saccharomyces cerevisiae). Activity assays indicate that, unlike most other mitochondrial phospholipases A, the yeast enzymes are Ca(2+)-independent with acidic (pH 4-5) as well as alkaline (pH 8-9) pH optima. Data obtained with mitochondria isolated from either fermenting or respiring cells, and initial observations with a petite strain, strongly suggest that a phospholipase A2 with an acidic pH optimum functions in the in vivo adaptation and maintenance of mitochondrial membranes required for respiration.
我们证明磷脂酶A1和磷脂酶A2都与分离出的酵母线粒体（酿酒酵母）相关。活性测定表明，与大多数其他线粒体磷脂酶A不同，酵母中的这些酶不依赖Ca(2+)，在酸性（pH 4 - 5）以及碱性（pH 8 - 9）的最适pH值下均有活性。从发酵细胞或呼吸细胞中分离出线粒体所获得的数据，以及对小菌落菌株的初步观察结果，都有力地表明，具有酸性最适pH值的磷脂酶A2在呼吸所需线粒体膜的体内适应性和维持过程中发挥作用。
