Jullien S, Capuozzo E, Salerno C, Crifò C
Department of Biochemical Sciences, University of Rome La Sapienza, Italy.
Biochem Int. 1991 May;24(2):307-19.
Membrane potential changes in human polymorphonuclear leukocytes have been studied by monitoring the fluorescence signal of a carbocyanine probe in the presence of calcium ionophore A23187, chemotactic tripeptide f-met-leu-phe, and polyene macrolides. Reduction of the signals triggered by the ionophore and the tripeptide, inhibition of the respiratory burst, and changes in the Na+ influx into the cell seem to be the dominant effect of the polyenes. Polyene-induced depolarization does not correlate with the inhibition of the respiratory burst.
通过监测在钙离子载体A23187、趋化三肽f-甲硫-亮-苯丙氨酸和多烯大环内酯存在下羰花青探针的荧光信号，研究了人类多形核白细胞中的膜电位变化。多烯类化合物的主要作用似乎是降低由离子载体和三肽触发的信号、抑制呼吸爆发以及细胞内钠离子流入的变化。多烯诱导的去极化与呼吸爆发的抑制无关。
