D-精氨酸对L-鸟氨酸摄取以及L-瓜氨酸和尿素生物合成的刺激作用。
Stimulation of L-ornithine uptake and L-citrulline and urea biosynthesis by D-arginine.
作者信息
Saavedra-Molina A, Piña E
机构信息
Departamento de Bioquímica, Facultad de Medicina, U.N.A.M., México, D.F.
出版信息
Biochem Int. 1991 May;24(2):349-57.
The action of D-arginine on isolated cells and mitochondria obtained from rat liver was studied. The D-amino acid at 200 microM stimulated by 40% the rate of urea biosynthesis by isolated hepatocytes. Citrulline formation was increased 60-70% in rat liver mitochondria incubated with 10 microM D-arginine. In these mitochondria, ornithine uptake was enhanced 204% with 1 microM D-arginine. Inhibition in urea and citrulline synthesis and in ornithine uptake was recorded with high concentrations of the D-amino acid. Respiratory control in liver mitochondria with glutamate-malate was inhibited 32% by 100 microM D-arginine. In isolated mitochondria loaded with Fluo-3-acetoxymethyl (AM) ester, 50 microM D-arginine diminished the matrix free calcium concentration.
研究了D-精氨酸对从大鼠肝脏分离得到的细胞和线粒体的作用。200微摩尔的D-氨基酸使分离的肝细胞尿素生物合成速率提高了40%。在与10微摩尔D-精氨酸一起孵育的大鼠肝脏线粒体中，瓜氨酸的生成增加了60 - 70%。在这些线粒体中，1微摩尔D-精氨酸使鸟氨酸摄取增强了204%。高浓度的D-氨基酸会导致尿素和瓜氨酸合成以及鸟氨酸摄取受到抑制。100微摩尔D-精氨酸使肝脏线粒体中谷氨酸-苹果酸呼吸控制受到32%的抑制。在加载了Fluo-3-乙酰氧基甲酯（AM）的分离线粒体中，50微摩尔D-精氨酸降低了基质游离钙浓度。
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