Fu Lei, Yun Francisco, Oczak Marko, Wong Betty Y L, Vieth Reinhold, Cole David E C
Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada M5G 1L5.
Clin Biochem. 2009 Jul;42(10-11):1174-7. doi: 10.1016/j.clinbiochem.2009.03.008. Epub 2009 Mar 18.
To determine the effect of vitamin D binding protein (DBP) genotypes on 25-hydroxyvitamin D [25(OH)D] changes with vitamin D supplements, we studied 98 adults receiving 600 or 4000 IU/d vitamin D(3) for one year.
The DBP functional variant, T436K, was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Mean 25(OH)D increases were 97% for TT (n=48), 151% for TK (n=31) and 307% (n=6) for KK genotypes (p=.004).
As with baseline 25(OH)D, T436K genotype predicts 25(OH)D changes after long-term vitamin D supplementation.
为了确定维生素D结合蛋白（DBP）基因型对补充维生素D后25-羟基维生素D[25(OH)D]变化的影响，我们对98名成年人进行了研究，这些成年人接受了为期一年的每天600或4000国际单位的维生素D3补充。
通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对DBP功能变体T436K进行基因分型。
TT基因型（n=48）的平均25(OH)D增加97%，TK基因型（n=31）增加151%，KK基因型（n=6）增加307%（p=0.004）。
与基线25(OH)D一样，T436K基因型可预测长期补充维生素D后25(OH)D的变化。
