利用SEREX技术鉴定肝细胞癌中的肿瘤相关抗原
Identification of tumor-associated antigens by using SEREX in hepatocellular carcinoma.
作者信息
Wang Kaijuan, Xu Xueqin, Nie Yifei, Dai Liping, Wang Peng, Zhang Jianying
机构信息
Department of Epidemiology, Zhengzhou University, China.
出版信息
Cancer Lett. 2009 Aug 28;281(2):144-50. doi: 10.1016/j.canlet.2009.02.037. Epub 2009 Mar 21.
AIM
To identify biomarkers for diagnosis and prognosis of hepatocellular carcinoma (HCC).
METHODS
Screening the HCC cDNA library with HCC patients sera. Isolated proteins were used as antigens to detect antibodies from patients with HCC and control sera.
RESULTS
Eighty-one positive clones were identified. The frequencies of autoantibody against five HCC-associated antigens were higher in HCC than that in chronic hepatitis and normal human sera. The sensitivity and specificity of KRT23, AHSG and FTL antigens combination tests up to 98.2% in joint test and 90.0% in series test separately.
CONCLUSIONS
HCC associate antigens identified from this study supply candidate markers of diagnosis, combined detection and immunotherapy of HCC.
目的
鉴定肝细胞癌（HCC）诊断和预后的生物标志物。
方法
用HCC患者血清筛选HCC cDNA文库。将分离出的蛋白质作为抗原，检测HCC患者和对照血清中的抗体。
结果
鉴定出81个阳性克隆。HCC患者血清中针对5种HCC相关抗原的自身抗体频率高于慢性肝炎患者和正常人血清。KRT23、AHSG和FTL抗原联合检测的敏感性和特异性分别在联合检测中高达98.2%，在串联检测中高达90.0%。
结论
本研究鉴定出的HCC相关抗原为HCC的诊断、联合检测和免疫治疗提供了候选标志物。
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