基于改性二氧化钛-牛血清白蛋白的纳米复合材料用于功能应用。

Nanocomposite based on modified TiO2-BSA for functional applications.

作者信息

Simi C K, Abraham T Emilia

机构信息

Chemical Sciences and Technology Division, National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram, India.

出版信息

Colloids Surf B Biointerfaces. 2009 Jul 1;71(2):319-24. doi: 10.1016/j.colsurfb.2009.02.019. Epub 2009 Mar 4.

DOI:10.1016/j.colsurfb.2009.02.019

PMID:19304461

Abstract

A novel nanocomposite based on TiO(2)-protein for functional applications was prepared and characterized. The composite was made by covalent immobilisation of bovine serum albumin (BSA) on to the TiO(2) particles. BSA was attached to the TiO(2) powder through a three-step process. This involves the functionalization of the TiO(2) particles with silane and the subsequent coupling by glutaraldehyde to free NH(2) groups of the protein. Formation of Schiff's base was confirmed by IR spectroscopy and the optimum loading of the BSA was found to be 73.61%. Surface morphology of the composite was studied by SEM and TEM. Thermal analysis of TiO(2)-BSA composite was carried out by TGA and DSC. Structural variation of the BSA after immobilisation was studied by CD spectra.

摘要

制备并表征了一种基于TiO(2)-蛋白质的新型纳米复合材料用于功能应用。该复合材料通过将牛血清白蛋白（BSA）共价固定在TiO(2)颗粒上制成。BSA通过三步过程附着到TiO(2)粉末上。这包括用硅烷对TiO(2)颗粒进行功能化，随后通过戊二醛与蛋白质的游离NH(2)基团偶联。通过红外光谱证实了席夫碱的形成，并且发现BSA的最佳负载量为73.61%。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）研究了复合材料的表面形态。通过热重分析（TGA）和差示扫描量热法（DSC）对TiO(2)-BSA复合材料进行了热分析。通过圆二色光谱（CD光谱）研究了固定化后BSA的结构变化。